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Proteomics Laboratory
Principle Investigator: Cheng, Yu-Che, Ph.D.


Current Affiliation

Research Fellow, Cathay Medical Research Institute

No. 32, Ln. 160, Jiancheng Rd., Xizhi Dist., New Taipei City, 22174, Taiwan

Tel: +886-26907965-2615, Fax: +886-26907963

E-mail: yccheng@cgh.org.tw

Adjunct Associate Professor

Department of Biomedical Science and Engineering

National Central University, Jhongli Dist., Taoyuan City, Taiwan



07/2006 Ph.D., Graduate Institute of Biochemistry and Molecular Biology, College of Medicine, National Taiwan University

07/1997 M.S., Graduate Institute of Agriculture Chemistry, National Taiwan University

06/1995 B.S., Department of Agriculture Chemistry, National Taiwan University


Professional Interests

Protein chemistry, Mass spectrometry and Mechanobiology


Professional Experience

2006 - present    Research Fellow, Cathay Medical Research Institute

2010 - 2018    Adjunct Assistant Professor, Graduate Institute of Biomedical Engineering

2018 - present    Adjunct Associate Professor, Department of Biomedical Science and Engineering

2003-2006    Operator, ESI-LC-MS/MS, Protein Core Lab, College of Medicine, National Taiwan University

2003-2006    Research Assistant, Program for Promotion Academic Excellence of Universities

2005-2013    Lecturer, Course of Proteomics, College of Bioresource, National ILan University

Honors / Awards

2006    Excellent paper award of the year, College of Medicine, National Taiwan University



  1. Cheng, Y. C., Hsueh, C. C., Lu, H. C. and Liao, T. H. (2006). Identification of three crucial histidine residues (His115, His132 and His297) in porcine deoxyribonuclease II. Biochemical J. 2006, 398(2):177-85.
  2. Huang C.J., Chien C.C., Yang S.H., Chang C.C., Sun H.L., Cheng Y.C., Liu C.C., Lin S.C., Lin C.M. Fecal ribosomal protein L19 is a genetic prognostic factor for survival in colorectal cancer. J Cell Mol Med. 2008, 12(5B):1936-1943
  3. Chen, W. J., Huang, P. T., Cheng, Y. C. and Liao, T. H. (2008) Putative secondary active site of bovine pancreatic deoxyribonuclease I. Protein and Peptide Letters.2008, 15:245-261
  4. Kao, D.Y., Cheng, Y. C., Kuo, T. Y., Lin, S. B., Lin, C. C., Chow, L. P., Chen, W. J. (2009) Salt responsive outer membrane proteins of Vibrio anguillarum serotype O1 as revealed by comparative proteome analysis. Journal of Applied Microbiology. 2009,106:2079-2085
  5. Huang, J.S., Lin, C. M., Cheng, Y. C., Hung K. L., Chien, C. C., Chen, S. K., Chang, C.J, Chen, C. W. & Huang, C. J. (2009) A vitronectin M381T polymorphism increases risk of hemangioblastoma in patients with VHL gene defect. J Mol. Med 2009, 87:613–622
  6. Wu, L.C., Chen, H.H., Horng, J.T., Lin, C, Huang, N.E., Cheng, Y.C., Cheng, K.F. A Novel Preprocessing Method Using Hilbert Huang Transform for MALDI-TOF and SELDI-TOF Mass Spectrometry Data. PLoS ONE 2010, 5(8):e12493
  7. Lin, J.Y.S., Cheng, Y.C., Chen, J.Y.R., Chien, C.C., Lin, S.C., Wen, R.T., Tsou, T.S., Ling, Q.D. Increased spinal prodynorphin gene expression in reinflammation- associated hyperalgesia after neonatal inflammatory insult. BMC Neuroscience 2010, 11:139
  8. Lin PH, Kao YH, Chang Y, Cheng YC, Chien CC, Chen WY. Daunomycin interaction with DNA: microcalorimetric studies of the thermodynamics and binding mechanism. Biotechnol J. 2010, 5(10):1069-77
  9. Chen, Y.W., Huang,T., Cheng, Y.C., Chien, C.C. and Tsao, C.W. Fabrication of nanostructured silicon by metal-assisted etching and its effects on matrix-free laser desorption/ionization mass spectrometry. Analytica Chimica Acta 2011, Feb 21;687(2):97-104
  10. Cheng, Y. C., Chen, K. H., Wang, J. S., Hsu, W. L., Chien, C. C., Chen, W. Y., and Tsao, C. W. Rapid Analysis of Abused Drugs Using Nanostructured Silicon Surface Assisted Laser Desorption/Ionization Mass Spectrometry. Analyst, 2012, Feb, 137: 654-661
  11. Tsao, C. W., Lin, C. H., Cheng, Y. C., Chien, C. C., Chen, W. Y., Nanostructured silicon surface modifications for as a selective matrix-free laser desorption/ionization mass spectrometry. Analyst, 2012, Jun 7;137(11):2643-50
  12. Hu W. W., Syu W. J., Chen W. Y., Ruaan R. C., Cheng, Y. C., Chien C. C., Li C., Chung C. A., Tsao C. W., Use of Biotinylated Chitosan for Substrate-Mediated Gene Delivery. Bioconjugate Chemistry, 2012 Aug 15;23(8):1587-99
  13. Guo S-L, Chen P-C, Chen M-S, Cheng Y-C, Lin J-M, et al. (2012) A Fast Universal Immobilization of Immunoglobulin G at 4°C for the Development of Array-based Immunoassays. PLoS ONE 7(12): e51370. doi:10.1371/journal.pone.0051370
  14. li>Shao-Kuan Chen, Chih-Ang Chung, Yu-Che Cheng, Chi-Jung Huang, Ruoh-Chyu Ruaan, Wen-Yih Chen, Chuan Li, Chia-Wen Tsao, Wei-Wen Hu, Chih-Cheng Chien, Hydrostatic pressure enhances mitomycin C induced apoptosis in urothelial carcinoma cells. Urol Oncol. 2014 Jan;32(1):26.e17-24. doi: 10.1016/j.urolonc.2012.09.004.
  15. Huang, Hsing-I; Chen, Shao-Kuan; Robert, Wang; Chia-Rui, Shen; Cheng, Yu-Che. Human Foreskin-derived Fibroblast-like Stromal Cells are able to Differentiate into Functional Hepatocyte-like Cells. Cell Biology International, 2013 Dec;37 (12) :1308-19.
  16. Wei-Wen Hu, Yung-Jen Chen, Ruoh-Chyu Ruaan, Wen-Yih Chen, Yu-Che Cheng, Chih-Cheng Chien. The regulation of DNA adsorption and release through chitosan multilayers. Carbohydrate polymers, 2014 Jan;99:394-402. doi: 10.1016/j.carbpol.2013.08.088. Epub 2013 Aug 29.
  17. Cherng CH, Lee KC, Chien CC, Chou KY, Cheng YC, Hsin ST, Lee SO, Shen CH, Tsai RY, Wong CS. Baicalin ameliorates neuropathic pain by suppressing HDAC1 expression in the spinal cord of spinal nerve ligation rats. J Formos Med Assoc. 2013 May 15. pii: S0929-6646(13)00134-4. doi: 10.1016/j.jfma.2013.04.007.
  18. Huang CJ, Yang SH, Lee CL, Cheng YC, Tai SY, Chien CC. Ribosomal protein S27-like in colorectal cancer: a candidate for predicting prognoses. PLoS One. 2013 Jun 24;8(6):e67043. doi: 10.1371/journal.pone.0067043.
  19. Guo SL, Han CT, Jung JL, Chen WJ, Mei JJ, Lee HC, Cheng YC*. Cystatin C in Cerebrospinal Fluid is Upregulated in Elderly Patients With Chronic Osteoarthritis Pain and Modulated Through Matrix Metalloproteinase 9-Specific Pathway. Clin J Pain. 2013 Jul 24. doi: 10.1097/AJP.0b013e31829ca60b (*Correspondence)
  20. Wei-Wen Hu, Yi-Ting Hsu, Yu-Che Cheng, Chuan Li, Ruoh-Chyu Ruaan, Chih-Cheng Chien, Chih-Ang Chung, Chia-Wen Tsao. Electrical Stimulation to Promote Osteogenesis using Conductive Polypyrrole Films. Mater Sci Eng C Mater Biol Appl. 2014 Apr 1;37:28-36.
  21. Cheng CC, Chung CA, Su LC, Chien CC, Cheng YC*. Osteogenic differentiation of placenta-derived multipotent cells in vitro. Taiwan J Obstet Gynecol. 2014 Jun;53(2):187-92. (*Correspondence)
  22. Chen SK, Chung CA, Cheng YC, Huang CJ, Chen WY, Ruaan RC, Li C, Tsao CW, Hu WW, Chien CC. Toll-Like Receptor 6 and Connective Tissue Growth Factor Are Significantly Upregulated in Mitomycin-C-Treated Urothelial Carcinoma Cells Under Hydrostatic Pressure Stimulation. Genet Test Mol Biomarkers. 2014 Jun;18(6):410-6.
  23. Yu-Che Cheng, Chia-Wen Tsao, Meng-Zhi Chiang, Chih-Ang Chung, Chih-Cheng Chien, Wei-Wen Hu, Ruoh-Chyu Ruaan, Chuan Li. Microfluidic platform for human placenta-derived multipotent stem cells culture and applied for enhanced neuronal differentiation. Microfluidics and Nanofluidics. 2014 Jul; DOI: 10.1007/s10404-014-1455-2 (*1st author)
  24. Tsai RY, Cheng YC, Wong CS. (+)-Naloxone inhibits morphine-induced chemotaxis via prevention of heat shock protein 90 cleavage in microglia. J Formos Med Assoc. 2015 May;114(5):446-55. doi: 10.1016/j.jfma.2014.12.004.
  25. Shao-Li Han, Meng-Jie Xie, Chih-Cheng Chien, Yu-Che Cheng, Chia-Wen Tsao Using MEMS-based inertial sensor with ankle foot orthosis for telerehabilitation and its clinical evaluation in brain injuries and total knee replacement patients Microsystem Technologies 22(3) · February 2015
  26. Shao‑Li Han, Meng‑Jie Xie, Chih‑Cheng Chien, YuChe Cheng and Chia‑Wen Tsao. Using MEMS-based inertial sensor with ankle foot orthosis for telerehabilitation and its clinical evaluation in brain injuries and total knee replacement patients. Microsyst Technol 22, 625–634 (2016). https://doi.org/10.1007/s00542-015-2439-1
  27. Chia-Wen Tsao, Yu-Che Cheng, and Jhih-Hao Cheng. Fluid Flow Shear Stress Stimulation on a Multiplex Microfluidic Device for Rat Bone Marrow Stromal Cell Differentiation Enhancement. Micromachines 2015, 6, 1996–2009; doi:10.3390/mi6121470
  28. Cheng YC, Huang CJ, Lee YJ, Tien LT, Ku WC, Chien R, Lee FK, Chien CC. Knocking down of Heat-shock Protein 27 directs differentiation of functional glutamatergic neurons from Placenta-derived Multipotent Cells. Rep. 2016, 6, 30314; doi: 10.1038/srep30314 (*Co-1st author)
  29. Chih-Cheng Chien, Chi-Jung Huang, Lu-Tai Tien, Yu-Che Cheng, Chia-Ying Ke, Yih-Jing Lee. Suppression of HSP27 Restores Retinal Function and Protects Photoreceptors From Apoptosis in a Light-Induced Retinal Degeneration Animal Model. Invest Ophthalmol Vis Sci. 2017 Jun 1;58(7):3107-3117. doi: 10.1167/iovs.16-21007.
  30. Sheng-Yuan Huang, Chih-Cheng Chien, Ruey-Shyang Hseu, Victoria Ying Jen Huang, Shang Ying Chiang, Chi-Jung Huang, Shao-Kuan Chen, Ru-Yin Tsai, Hsi-Ting Lin, Yu Che Cheng. Ganoderma microsporum immunomodulatory protein induces apoptosis and potentiates mitomycin C-induced apoptosis in urinary bladder urothelial carcinoma cells. J Cell Biochem. 2017 Dec 14. doi: 10.1002/jcb.26616. (*Correspondence)
  31. Yu-Hsiang Lee, Chia-Wei Lai, Yu Che Cheng. Fluid Shear Stress Induces Cell Cycle Arrest in Human Urinary Bladder Transitional Cell Carcinoma Through Bone Morphogenetic Protein Receptor-Smad1/5 Pathway. Mol. Bioeng. 2018 June, Vol. 11 (3), pp. 185–195 doi: 10.1007/s12195-018-0523-1 (*Co-Correspondence)
  32. Wei-Wen Hu, Tun-Chi Chen, Chia-Wen Tsao, and Yu-Che Cheng *The effects of substrate-mediated electrical stimulation on the promotion of osteogenic differentiation and its optimization. Journal of Biomedical Materials Research: Part B - Applied Biomaterials. 2018 Oct 14. doi: 10.1002/jbm.b.34253. (*Correspondence)
  33. Yu-Che Cheng*, Ru-Yin Tsai, Yen-Tseng Sung, Ing-Jung Chen, Tzu-Yi Tu, Ya-Yuan Mao, and Chih-Shung Wong. Melatonin regulation of transcription in the reversal of morphine tolerance: Microarray analysis of differential gene expression. International Journal of Molecular Medicine. Vol. 43: pp.791-806, 2019, doi: 10.3892/ijmm.2018.4030. (*Co-1st author)
  34. Yu-Che Cheng*, Chien-Hsun Chen, Hong-Wei Kuo, Ting-Ling Yen, Ya-Yuan Mao, and Wei-Wen Hu. Electrical Stimulation through Conductive Substrate to Enhance Osteo-Differentiation of Human Dental Pulp-Derived Stem Cells. Sci. 2019, 9, 3938; doi:10.3390/app9183938. (*Co-1st author)
  35. Yu-Che Cheng*, Shu-Lin Guo, Kun-Da Chung and Wei-Wen Hu. Electrical Field-Assisted Gene Delivery from Polyelectrolyte Multilayers. Polymers 2020, 12, 133; doi:10.3390/polym12010133. (*Co-1st author)
  36. Yu-Che Cheng*, Wei-Chi Ku 1, Ting-Ting Tseng, Ching-Po Wu, Meng-Jin Li, Shao-Chen Lee. Anchorage independence altered vasculogenic phenotype of melanoma cells through downregulation in aminopeptidase N /syndecan-1/integrin β4 axis. Aging (Albany NY). 2020 Aug 4;12(17):16803-16819. doi: 10.18632/aging.103425. (*1st author)
  37. Chia-Yu Shih and Yu-Che Cheng*, Chiao-Hui Hsieh, Ting-Ting Tseng, Shih-Sheng Jiang, Shao-Chen Lee. Drug-selected population in melanoma A2058 cells as melanoma stem-like cells retained angiogenic features – the potential roles of heparan-sulfate binding ANGPTL4 protein. Aging (Albany NY). 2020 Nov 10;12. doi: 10.18632/aging.103890. (*Co-1st author)
  38. Wen-Chin Ko, Chia-Ti Tsai, Kai-Cheng Hsu, Yu-Che Cheng, Tony Eight Lin, Yi-Ling Chen, Chuang-Ye Hong, Wan-Jung Lu, Chun-Ming Shih and Ting-Lin Yen. Magnolol, A Novel Antagonist of Thrombin and PAR-1, Inhibits Thrombin-Induced Connective Tissue Growth Factor (CTGF) Expression in Vascular Smooth Muscle Cells and Ameliorate Pathogenesis of Restenosis in Rats. Sci. 2020, 10(23), 8729; https://doi.org/10.3390/app10238729 - 05 Dec 2020.



2014 –2015 NSC 103-2320-B-281-001- (PI), NTD: 1,173,000

2017 – 2020 MOST 106-2314-B-281-002-MY3 (Co-PI), NTD 4,436,000

2017 –2020 MOST 107-2314-B-281-001-MY3 (PI), NTD: 4,055,000

2018 –2020 MOST 108-2221-E-008-028-MY2- (Co-PI), NTD: 2,070,000

2020 –2023 MOST 109-2314-B-281-002-MY3 (Co-PI), NTD: 4,155,000